STUDIES ON DEGRADATION OF ANTIGENICITY OF PPR VACCINE AND ASSESSMENT OF ITS MATERNAL ANTIBODY. Sayam ASM, Rahman MB, Islam MR, Ramam MM, Hoque N

ABSTRACT

The degradation procedure of antigenecity of PPR vaccine after reconstitution was studied in goats at farmers’ level with seven groups of animals and each group had four animals at Isagram, Mymensingh, the mean antibody titre±SD calculated with CELISA of sera samples collected at pre-vaccination, at 14th and 28th day of post vaccination were 36.92%, 23.37%, 24.33%, 34.23%, 23.56%, 30.69%, 15.42%; 60.91%, 59.47%, 58.97%, 57.05%, 55.16%, 41.01%, 36.69% and 72.77%, 71.37%, 69.74%, 60.24%, 55.41%, 46.12%, 35.31% in respective to 0, 0.5, 1, 1.5, 2, 2.5, and 3 hours interval. Similarly, at Myzebari, Mymensingh, the mean antibody titre±SD at pre vaccination, 14th and 28th day of post vaccination were 26.90%, 24.99%, 26.33%, 17.73%, 30.57%, 28.03%, 24.99%; 61.17%, 58.41%, 57.94%, 49.29%, 51.48%, 34.01%, 57.94%, 49.29%, 51.48%, 34.01%, 28.88% and 72.36%, 65.08%, 63.85%, 57.85%, 54.29%, 42.84%, 24.50% in respective to 0, 0.5, 1, 1.5, 2, 2.5 and 3 hours interval. Both the trail shows antigenicity degrades gradually with time after reconstitution of vaccine which may be due to the exposure of vaccine to temperature, light and reconstituent (PBS). On the other hand, assessment of maternal antibody was done collecting sera samples from kids and their respective dams from Government goat farm and BLRI goat farm Savar, Dhaka. All the dams had been vaccinated every 6 months for the past two years. At Government goat farm the kids were divided into 5 age groups, 10-30 days, 31-60 days, 61-90 days, 91-120 days and 121-160 days. The mean antibody titre±SD estimated with C-ELISA of respective age groups of kids and respective dams at Government goat farm, Savar were 54.69%, 53.99%, 52.86%, 52.73%, 40.17%, and 67.58%, 63.73%, 66.71, 66.69%, 59.02%. At BLRI farm the kids were divided into 3 age groups 10-30 days, 31-60 days, and 61-90 days. The mean antibody titre±SD of kids in these groups were 54.09%, 50.93%, 50.15% and respective dams were 66.66%, 63.59%, 66.51%. From the above study it could be concluded that up to 4 months of age of kids of the vaccinated dams, the maternal antibody remains above 50%. So kids should be vaccinated after 4 months of age.

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OPTIMIZATION OF DOSE OF 17 Α-METHYL TESTOSTERONE HORMONE AND EFFECT OF STOCKING DENSITY ON THE GROWTH PERFORMANCE OF MONOSEX NILE TILAPIA (Oreochromis niloticus). Akter S, Alam MJ, Sharker MR, Ali MM

ABSTRACT

 The present experiment was conducted with a view to assessing the optimum dose of 17α-methyl testosterone hormone to produce all male tilapia and effects of stocking density on the growth performance and production of monosex tilapia (Oreochromis niloticus) in hapa for a period of 120 days at Authentic Matshya Hatchery, Mymensingh. After preparing the pond seven days old fry of tilapia (O. niloticus) were stocked at the rate of 4500, 4000, and 5000 fish fry hapa-1 in three treatments of T1, T2 and T3 with mean initial weight of 0.009g. During the trial period of 28 days 60mg MT Kg-1, 55mg MT kg-1, 65mg MT kg-1 hormone mixed feed were used to produce monosex male tilapia. The nursing of the experimental fry was continued for further 3 months with commercial mega feed. At the end of experiment the sex ratio was determined by aceto carmine gonad squashing method. The result of the study showed that fry in treatment T2 exhibit the highest mean weight gain, specific growth rate (SGR day-1), highest survival rate than T1 and T2 treatments. Water quality parameters such as temperature, dissolved oxygen, pH and transparency were found to be within suitable range throughout the experimental period. The mean percentage of male obtained in the experiment 91.43%, 94.28% 90.43% at doses of 60, 55 and 65mg 17α- methyl testosterone per kg of feed respectively. The dose of 55 mg 17α- methyl testosterone per kg of feed in treatment T2 was found to be best for sex reversal in O. niloticus resulting 94.28% male in the population and the growth rate is highest at the density of 4000 fish hapa-1.

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EFFECT OF REDUCED RATES OF N AND P ON BINAdhan-7 AT OLD BRAHMAPUTRA FLOODPLAIN SOIL. Haque ANA, Tarafder MA, Haque MA, Haque ME and Hashem MA

ABSTRACT

An experiment was conducted at Mymensingh during aman season 2011 to study the response of Binadhan-7 to reduced rates of nitrogen and phosphorus. The experiment was laid out in a randomized complete block design (RCBD) with three replications. There were eight treatments as T1 (control), T2 (RFD), T3 (RFD -25% N), T4 (RFD – 50% N), T5 (RFD – 25% P), T6 (RFD – 50% P), T7 (RFD – 25% NP) and T8 (RFD – 50% NP). 80 kg N ha-1, 25 kg P   ha-1, 35 kg K ha-1, 10 kg S ha-1 and 4 kg Zn ha-1 were applied as recommended fertilizer dose (RFD). Nitrogen, phosphorus, potassium, sulphur and zinc were supplied through urea, TSP, MOP, gypsum and zinc sulphate, respectively. The full dose of TSP, MOP, gypsum and zinc sulphate was applied as basal dose during final land preparation while urea was applied in three equal splits. The grain and straw yield of Binadhan-7 were significantly affected due to different treatments. The highest grain yield of 5.09 t ha-1 and straw yield of 7.01 t ha-1 were recorded in the treatment T2. Grain yield was positively correlated with plant height, tillers hill-1, panicle length, filled grains panicle-1 and 1000-grain weight whereas negatively correlated with unfilled grain panicle-1. The N and P content and uptake by rice plant did not decrease considerably due to the reduced rates of N and P compared to RFD.

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AMPLIFICATION AND CLONING OF FULL LENGTH HEMAGGLUTININ GENE OF A LOW PATHOGENIC (H9N2) AVIAN INFLUENZA VIRUS. Hasan Mehedi, Haque ME, Begum JA and Ali M

ABSTRACT

Avian influenza is an infectious disease of birds caused by influenza type A virus belonging to the family Orthomyxoviridae. Long-term co-circulation of multiple virus lineages (e.g., H5N1 and H9N2 viruses) in different types of poultry has facilitated the frequent reassortment in H5N1 and H9N2 influenza viruses. This situation favors the emergence of influenza viruses with pandemic potential. Therefore it is important to know the detail molecular epidemiology of Bangladeshi LPAI H9N2 subtype. The objective of this study was the amplification and cloning of full length hemagglutinin gene of a low pathogenic H9N2 avian influenza virus. A full length hemagglutinin gene (1778 bp) of low pathogenic avian influenza was amplified by RT-PCR using HA (H9) gene specific primer. The amplicon was cloned in pGEM®-T Easy Vector (Promega, USA). The cloned cDNA was purified and sent to a commercial laboratory for sequencing. Phylogenetic analysis based on complete hemagglutinin (H9) gene sequence revealed that the Bangladeshi isolate of H9N2 LPAI is closely related to Indian isolates. Along with other isolates from South Asia and Middle East the Bangladeshi isolates belonged to A/Qauil/HK/G1/97(H9N2) like sub-lineage of H9N2 viruses.

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GROWTH DYNAMICS OF SOME SELECTED GERMPLASM OF SOYBEAN IN TWO PLANTING SEASONS OF BANGLADESH. Monshi FI, Malek MA and Tabassum R

ABSTRACT

An experiment was conducted at Mymensingh, Bangladesh during kharif-II season of 2009 and rabi season of 2010. Eight selected germplasm of soybean (BAU-S/6, BAU-S/101, BAU-S/109, BAU-S/117, BAU-S/131, BAU-S/136 and BAU-S/158) with check variety Shohag were planted to evaluate their performance of growth and yield in response to planting seasons. The characters studied were: plant height, branches per plant, pod plant-1, pod length, seeds pod-1, 100-seed weight, days to flowering, days to maturity and seed yield per hectare. Results showed significant variation among the germplasm lines and check variety for all the characters both in two different planting seasons. Robi season showed higher plant height (38 to 88 cm) than that of kharif II season (29 to 60 cm) along with longer flowering and maturity period. In average, pods plant-1 (31 to 57), seeds pod-1 (1.6 to 2.4) and 100-seed weight (9.12 to 13.89) performed better in robi season than that of kharof II obtaining 33 to 49, 1.6 to 2.7 and 9.60 to 13.55, respectively. Soybean germplasm BAU-S/136 produced the highest seed yield of 3641 kg ha-1 in robi and 3420 kg ha-1 in kharif II season followed by BAU-S/158 (3259 kg ha-1 in kharif II and 3428 kg ha-1 in robi) and BAU-S/109 (3125 kg ha-1 in kharif II and 2913 kg ha-1 in robi) considering other yield attributing characters both in two planting seasons and robi season also showed higher performance than that of kharif II. Promising lines BAU-S/136, BAU-S/158 and BAU-S/109 are needed further trial for conformity of their genetic efficiency present among these lines of soybean in Bangladesh.

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PLANKTONIC BIODIVERSITY OF THE DHEPA RIVER IN BANGLADESH. Ferdoushi Zannatul and Rakiba Kazi

ABSTRACT

 This article presents the planktonic biodiversity of the Dhepa River in Dinajpur district of Bangladesh. The research was conducted from September 2011 to April 2012 in six different sampling sties of the Dhepa River. A total of 31 species of phytoplankton were identified. Dominant phytoplankton group was Bacillariophyceae followed by Chlorophyceae, Cyanophyceae and Euglenophyceae. The phytoplankton biomass showed mainly two peaks in the present study, first peak in January and the second in April. Among 10 identified species of zooplankton Rotifer was the most dominant group. Like phytoplankton, zooplankton also showed two peaks, first in September and second in January. However, the overall plankton production reached to the highest level in the months of December to January. It might be due to low level of water depth and nutrient concentration of the river water.

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EFFECT OF MAGNESIUM, ZINC AND BORON ON YIELD AND NUTRIENT UPTAKE BY WHEAT. Tarafder MA, Das S, Tasmin S and Khan MR

ABSTRACT

Quantitative information regarding effects of Magnesium, Boron and Zinc in wheat (Triticum aestivum) is scarce. Application of these nutrients can ensure the availability of nutrients to crops for obtaining higher yield. To study the effect of magnesium, zinc and boron on yield and nutrient uptake by wheat, a field experiment was conducted at Birgonj, Dinajpur under the Old Himalayan Piedmont plain soil during rabi season of 2013 with wheat crop (var. Prodip) to evaluate the optimum and economic rate of Mg, Zn and B. The experiment was designed with eight treatments and carried out in a randomized complete block design (RCBD) with three replications. The treatment combinations were T1: (control), T2: Mg, T3: Zn, T4: B, T5: Mg+B, T6: Zn+B, T7: Mg+Zn and T8: Mg+Zn+B. Mg, Zn and B were applied @ 6, 2 and 1 kg ha-1 respectively. All the plots received an equal rate of N, P, K and and S (N100 P25 K75 S13) to support normal plant growth. The grain and straw yields of wheat were significantly influenced due to the application of different treatment combinations of Mg, Zn and B. The grain yield varied from 3.18 to 4.35 t ha-1. The highest grain (4.35 t ha-1) was recorded in the treatment T8 with the combined application of Mg, Zn and B which gave 27% higher yield over control followed by the treatment T7. Mg, Zn and B uptake by wheat crop were significantly influenced by different treatments. The highest nutrient uptake by both grain and straw was obtained from the treatment T8 and the lowest was obtained in control. The findings from the present study revealed that Mg, Zn and B along with NPKS are essential to obtained higher yield of wheat crop than that of the application of NPKS alone.

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EFFECT OF SPACING AND PHOSPHORUS ON GROWTH AND FLOWERING OF GLADIOLUS. Rajib RR, Naznin A, Shanta FH, Haque ANA, Amin MR

ABSTRACT

The field experiment was conducted at Dhaka, Bangladesh during the period from October 2011 to May 2012. The experiment consisted with two factors A: Three levels of spacing (S1, S2 & S3) and factor B: Four levels of phosphorus (P0, P1, P2 and P3). The result revealed that the highest number of spike (2,77,000 ha-1) was  recorded from spacing S2 and the lowest from spacing S1. In case of phosphorus, the highest number of spike (2,86,000 ha-1) was recorded from P2 and the lowest (189000 ha-1) was found from at P0. For interaction effect the highest number of spike (3,30,000 ha-1) was recorded from S2P2. So, the combination of 30 cm × 25 cm spacing with 150 kg P2O5 ha-1 is better for growth and flowering of gladiolus.

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ON-FARM EVALUATION THROUGH MODERN AND TRADITIONAL CULTIVATION PRACTICES OF POTATO PRODUCTION. Tabassum N, Haque MM, Bir MSH, Zaman MK and Shaha M

ABSTRACT

 The study was carried out using modern inputs against traditional inputs and practices for potato production were conducted in the different agro-ecological zones and agro-climatic locations of Bangladesh during 2011-2012. The selected study areas were brought under potato production with the different modern varieties of potato like Diamant (55%), Cardinal (30%), and Granola (15%). It was observed that the total cost of potato production (fixed cost and variable cost) varied from Tk. 132,180 to Tk. 148,735 per ha for modern to traditional cultivation practices. The major cost involvement for modern practices was observed in (a) seed (43.1%), (b) hired and family human labors (19.93%), (c) fertilizer (16.23%), and (d) pesticides (5.26%). And for traditional practices of potato the cost involvement was found (i) seed (46.46%), (ii) fertilizer (21.66%), (iii) hired and family human labors (10%) and (iv) pesticides (9.9%). The cost was comparatively low in modern practices than in traditional practices as it was found mainly due to adoption of recommended technology, appropriate dose and timely use of modern inputs and practices. The yield was found 26 t ha-1 in modern practices and 24 t/ha in traditional practices. The net profit was significantly higher in modern practices than traditional practices; the net profit in modern practices was found at Rangpur Tk. 98,079 per ha, Thakurgaon Tk. 72,741 per ha and Comilla Tk. 46,950 per ha. It was recommended from the study that the farmers will be economically benefited if they use modern inputs; adopt technologies; use quality seed of improved varieties at recommended dose; timely use of fertilizers and pesticides; timely application of irrigation; and appropriate crop management including weeding and other practices.

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A COMPARATIVE STUDY ON LIVELIHOOD OF RURAL WOMEN RELATED WITH GOAT REARING IN SELECTED AREAS OF BANGLADESH. Alam MM, Rokonuzzaman M and Yeasmin F

ABSTRACT

The study was conducted in two upazila namely Shaharasti and Hazigonj under Chandpur district during the period of June to July /2009  to analyze the socio-economic status of rural women in goat rearing.  For achieving the objectives total 30 farmers were selected for the study. From the study it revealed that yearly goat rearing cost were in Shaharasti & Hajigonj Tk.2550.00 & Tk. 2180.00 whereas, yearly return from goat rearing in Shaharasti & Hajigonj were Tk.3350.00 & Tk.2840.00, respectively. On the other hand, BCR of goat farming in Shaharasti was 1.31 and in Hajigonj was 1.30 considering one lactation period. The result clearly indicated that goat farming was profitable. The result clearly indicated that the reproductive performances of Black Bengal goat were better than cross bred goat. On the other hand, productive performances like higher birth weight, maximum body weight gain, high milk yield, longer lactation period were suitable in cross bred goat.

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