An experiment was conducted at Mymensingh, Bangladesh during kharif-II season of 2009 and rabi season of 2010. Eight selected germplasm of soybean (BAU-S/6, BAU-S/101, BAU-S/109, BAU-S/117, BAU-S/131, BAU-S/136 and BAU-S/158) with check variety Shohag were planted to evaluate their performance of growth and yield in response to planting seasons. The characters studied were: plant height, branches per plant, pod plant-1, pod length, seeds pod-1, 100-seed weight, days to flowering, days to maturity and seed yield per hectare. Results showed significant variation among the germplasm lines and check variety for all the characters both in two different planting seasons. Robi season showed higher plant height (38 to 88 cm) than that of kharif II season (29 to 60 cm) along with longer flowering and maturity period. In average, pods plant-1 (31 to 57), seeds pod-1 (1.6 to 2.4) and 100-seed weight (9.12 to 13.89) performed better in robi season than that of kharof II obtaining 33 to 49, 1.6 to 2.7 and 9.60 to 13.55, respectively. Soybean germplasm BAU-S/136 produced the highest seed yield of 3641 kg ha-1 in robi and 3420 kg ha-1 in kharif II season followed by BAU-S/158 (3259 kg ha-1 in kharif II and 3428 kg ha-1 in robi) and BAU-S/109 (3125 kg ha-1 in kharif II and 2913 kg ha-1 in robi) considering other yield attributing characters both in two planting seasons and robi season also showed higher performance than that of kharif II. Promising lines BAU-S/136, BAU-S/158 and BAU-S/109 are needed further trial for conformity of their genetic efficiency present among these lines of soybean in Bangladesh.