The field experiment was conducted at Dhaka, Bangladesh during the period from October 2011 to May 2012. The experiment consisted with two factors A: Three levels of spacing (S1, S2 & S3) and factor B: Four levels of phosphorus (P0, P1, P2 and P3). The result revealed that the highest number of spike (2,77,000 ha-1) was recorded from spacing S2 and the lowest from spacing S1. In case of phosphorus, the highest number of spike (2,86,000 ha-1) was recorded from P2 and the lowest (189000 ha-1) was found from at P0. For interaction effect the highest number of spike (3,30,000 ha-1) was recorded from S2P2. So, the combination of 30 cm × 25 cm spacing with 150 kg P2O5 ha-1 is better for growth and flowering of gladiolus.