Quantitative information regarding effects of Magnesium, Boron and Zinc in wheat (Triticum aestivum) is scarce. Application of these nutrients can ensure the availability of nutrients to crops for obtaining higher yield. To study the effect of magnesium, zinc and boron on yield and nutrient uptake by wheat, a field experiment was conducted at Birgonj, Dinajpur under the Old Himalayan Piedmont plain soil during rabi season of 2013 with wheat crop (var. Prodip) to evaluate the optimum and economic rate of Mg, Zn and B. The experiment was designed with eight treatments and carried out in a randomized complete block design (RCBD) with three replications. The treatment combinations were T1: (control), T2: Mg, T3: Zn, T4: B, T5: Mg+B, T6: Zn+B, T7: Mg+Zn and T8: Mg+Zn+B. Mg, Zn and B were applied @ 6, 2 and 1 kg ha-1 respectively. All the plots received an equal rate of N, P, K and and S (N100 P25 K75 S13) to support normal plant growth. The grain and straw yields of wheat were significantly influenced due to the application of different treatment combinations of Mg, Zn and B. The grain yield varied from 3.18 to 4.35 t ha-1. The highest grain (4.35 t ha-1) was recorded in the treatment T8 with the combined application of Mg, Zn and B which gave 27% higher yield over control followed by the treatment T7. Mg, Zn and B uptake by wheat crop were significantly influenced by different treatments. The highest nutrient uptake by both grain and straw was obtained from the treatment T8 and the lowest was obtained in control. The findings from the present study revealed that Mg, Zn and B along with NPKS are essential to obtained higher yield of wheat crop than that of the application of NPKS alone.